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Wednesday, May 13, 2020 | History

2 edition of Culture of mammary epithelial cells from lactating mice found in the catalog.

Culture of mammary epithelial cells from lactating mice

Polly Rae Cline

Culture of mammary epithelial cells from lactating mice

morphology and lactose synthesis

by Polly Rae Cline

  • 368 Want to read
  • 1 Currently reading

Published .
Written in English

    Subjects:
  • Cell culture.,
  • Mammary glands.,
  • Epithelium -- Effect of drugs on.

  • Edition Notes

    Statementby Polly Rae Cline.
    The Physical Object
    Paginationviii, 60 leaves :
    Number of Pages60
    ID Numbers
    Open LibraryOL16513183M

    HuMEC medium is a serum-free medium engineered specifically for the culture of Human Mammary Epithelial Cells (HMEC). HuMEC medium supports superior cell growth in the first culture after thaw and throughout the guaranteed lifespan, when compared to a major competitors' culture . in the development of the lactating mammary gland and in cultured HC11 cells (6, 7, 9–13). XOR is induced in mammary gland epithelial cells late in pregnancy, further stimulated by lactation, and then precipitously lost during involution (6, 10, 11). In cultured HC11 cells, XOR can be induced by the lactogenic hormones cortisol (or dex-Cited by:

    TRACKING MAMMARY EPITHELIAL CELL LINEAGE AND CELL DIVISIONS IN THE NORMAL MAMMARY GLAND AND MAMMARY NEOPLASIA USING TRANSGENIC MICE A Dissertation in Genetics by Jessica L. Mathers Jessica L. Mathers Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy August Author: Jessica L. Mathers. Therefore, the use of a primary mammary cell line is much more representative of the in vivo system (Pantschenko et al., ). Such primary mammary cell culture models can be used for lactation studies, in vitro expression of recombinant proteins, or to evaluate expression of gene constructs in mammary cells, prior to generating transgenic.

    replenishment of the prolactin receptor on mammary cells of lactating mice (13). Moreover, casein (14) and its mRNA (8) appear in the mammary gland in vivo as early as midpreg- nancy, indicating that the pregnancy mammary cells are under active stimulation of the lactogenic hormones. Consequently. Primary mouse mammary epithelial culture protocol. Primary culture protocol. adapted from Pullan, S.E. and Streuli, C.H. (). The mammary gland epithelial cell. In Epithelial cell culture Harris, A. (ed., pp Cambridge University Press, Cambridge, UK You get roughly about gm-2gm per mouse based on the age of mice and the number Dissecting tools: Autoclaved.


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Culture of mammary epithelial cells from lactating mice by Polly Rae Cline Download PDF EPUB FB2

Mammary epithelial cells from lactating mice synthesize and secrete lactose in culture and retain many features of their in vivo morphology if mammary glands are only partially dissociated to alveoli, rather than completely dissociated to single cells. After 5 d in culture lactose synthesis by alveoli cultured on floating collagen gels is 10 to 20 times higher than in cultures of single cells on floating collagen by: Incorporation of [UC]glucose into metabolites and metabolic products in mammary cells from virgin, pregnant and lactating mice was measured.

Rates of glycogen and lactose synthesis and ratios of lactate to alanine and of citrate to malate were important Author: J.

Emerman, J. Bartley, M. Bissell. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium.

DISCUSSION The successful culture of mammary epi- thelial cells derived from lactating mouse mammary glands appears to require gentler handling procedures than those routinely used in culturing cells from prelactating by:   Recent advances have enabled the isolation, molecular characterisation and functional analysis of mouse mammary epithelial cell subpopulations from the normal gland, including subpopulations enriched for stem cell by: ANALYSIS OF MURINE MAMMARY EPITHELIAL CELLULAR HIERARCHY by Neethan A.

Lobo A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy (Cellular and Molecular Biology) in The University of Michigan Doctoral Committee: Professor Charles Burant, Co-Chair.

6 Cell Biology of the Lactating Mammary Epithelium RNA and 13% microRNA. They are thought to interact with intestinal cells and regulate intestinal development and immunity in the infant.

McCaffrey Lab - Isolation of Primary Mouse Mammary Epithelial Cells 1. Euthanize mice with Isoflurane and cervical dislocation (see animal SOP _01, Section ). Isolate 2nd, 3rd, 4th, and 5th pairs of mammary glands. • Clean outside of mouse with 70% ethanol.

• Pin each paw to a surgical platform. Lineage tracing analysis allows mammary epithelial cells to be tracked in their natural environment, thereby revealing cell fate and proliferation choices in the intact tissue.

This technique is Cited by: 3. Isolation, Culture, and Differentiation of Mammary Epithelial Stem/Progenitor Cells from Fresh or Ex Vivo Cultured Human Breast Tissue Guang Chen Department of Cell Systems & Anatomy, School of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TexasAuthor: Guang Chen, Guang Chen, Hakim Bouamar, Lu‐Zhe Sun.

The main function of the mammary gland is to produce milk to sustain offspring. Here, the authors show that secretory alveolar cells in the lactating gland in several species are binucleated Cited by: anterior depot of lactating mice the mammary alveoli lie close to interscapular brown fat.

To assess whether, during mammary gland involution, alveolar epithelial cells transdifferentiate not only to white adipocytes, but also to brown adipocytes, we conducted an ultrastructural and lineage-tracing study.

MATERIALS AND METHODS Animals and tissues. Isolation and culture of mouse and human mammary organoids Mouse mammary epithelial cells were prepared as previously described (Stingl et al., ). The mammary glands from 2-week (wk) old mice were minced and digested overnight at 37°C in EpiCult B Mouse Medium (StemCell.

Normal mouse mammary epithelial cells from Balb/c mice were successfully cultivated on tissue culture plastic with lethally irradiated LA7 feeder cells.

The feeder cells also promoted colony formation from single mouse mammary cells, and the fraction of cells that formed colonies was proportional to the density of feeder by: The epithelial cell-specific effects of prolactin and epidermal growth factor (EGF) on the development of normal rat mammary epithelial cells (MEC) were evaluated using a three dimensional primary.

Culture of Mouse Mammary Epithelial Cells Mouse mammary epithelial cell cultures should be initiated from single-cell suspensions (refer to Section ).

Otherwise, cells will not adhere well to the tissue culture flask. Seed mouse mammary cells into tissue culture flasks at a density of 2 - 4 x cells/cm2 cells/well. Epithelial Cells. However, culture of mammary explants from pregnant mice in the presence of insulin, cortisol, and PRL before isolation of the epithelial cells results in a % increase in the basal rate of carrier-mediated glucose transport in the absence of exogenous hormones; this effect requires 3 days of by:   Insulin binding to its receptors was studied in collagenase-dissociated mammary cells prepared from mice that were cycling, pregnant, or lactating.

Mammary cells (3 × 10 6) were incubated in medium ( ml) supplemented with iodinated insulin (–1 ng/ml) for 45 min at room temperature. The dissociation of iodinated insulin from its Cited by: Electron microscopic studies demonstrate that approximately 3 - 8% of all mammary epithelial cells do not have a terminally differentiated phenotype, and are considered to reside higher within the mammary epithelial cell hierarchy.

1,2 For instance, it is thought that ER- luminal cells in the virgin mammary gland are progenitor cells that proliferate during pregnancy to generate alveoli; those cells in the alveoli are then capable of producing milk during lactation.

Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers.

In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the. It has been believed that the immunoglobulin (Ig) found abundantly in the colostrum of lactating mammalian is derived from serum or secreted by plasma cells present in the mammary gland.

The recent finding of Ig gene rearrangements in breast cancer cells and benign hyperplastic breast epithelial cells suggests that it is likely that hyperplastic mammary gland epithelial cells during lactation Cited by: Prolactin receptors were monitored by measuring I-labeled prolactin binding to collagenase-dissociated mammary epithelial cells of lactating BALB/c mice.

Specific receptors for iodine-labeled prolactin with an apparent dissociation constant (K d) of 10 −9 M were present on the dissociated mammary binding was inhibited by ovine prolactin, human growth hormone and human Cited by:   Mouse mammary tumour virus envelope proteins transform normal human mammary epithelial cells into invasive breast cancer cells.

in mice or mouse mammary tumour development. lactating women Author: James S. Lawson, Wendy K. Glenn.